Optimization of biogas production from a given substrate and digester is an issue that needs to be addressed during the development of anaerobic digestion. To maximize the biogas production rate, the operating parameters that influence anaerobic digestion must be controlled and monitored. The research was carried out using a 0.15 m3 laboratory digester. The study was aimed at evaluating the effect of different cow dung and maize silage mix ratios of 1:1; 1:3 and 3:1 which were compared to pure substrates at a constant temperature of 20℃, temperature (20°C, 25°C and 30°C with 3:1 feedstock mix ratio) and the stirring of 100 rpm for minutes was varied at different intervals of 1 hour, 2 hours, 6 hours and 12 hours with no stirring as a control.

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